WebThis effect appears mainly when HILIC and RPLC are coupled as the weak solvent used in HILIC is a strong eluent solvent in RP, and vice versa, leading to poor focusing of the analytes in the 2 D column head. One of the most frequently employed strategies to eliminate this incompatibility is online dilution with a weak solvent using trapping ... WebJun 21, 2024 · Hydrophilic interaction liquid chromatography (HILIC), in the absence of ion-pair reagents, has been investigated here as an alternative chromatographic approach to the analysis of oligonucleotides. By combining a mobile phase system using commonly employed in liquid chromatography-mass spectrometry (LC-MS) - i.e., water, acetonitrile, …
The Use of HILIC Zwitterionic Phase Superficially Porous Particles …
WebHILIC is generally recommended for compounds with negative log P values. Another important analyte parameter is the pK a (-log K a) value; K a gives us an indication of the acidity of a molecule. The larger the pK a value, the weaker the acid. log D provides a good indication of the ionization state of a compound in solution at a specific pH. WebHello HILIC… Protecting your laboratory productivity Recommended Reading Sample Prep Pointers - Peptide Mapping Part I Save your results with sample filtration Simplified cone inspection with the new Agilent LED measuring magnifier Software - Supported Method Development - The Scanview Application Software tool for the ADM Flow Meter (G6691A) the race of your life
Agilent Zorbax HILIC Plus
WebHILIC is a chromatographic technique that requires good method development in order to successfully separate difficult samples. When a HILIC protocol is set up properly, separations are highly effective and consistent. There are several important factors to consider when developing your HILIC method: Water Web12 contract might be guilty of misrepresentation (whether negligent or willful) or being unworthy or incompetent to act as a real estate broker, both violations of License Law … WebMar 19, 2024 · The applications of HILIC across each field include: Pharmacology: To analyze drugs and their metabolites. Biochemistry: Metabolic pathways and biomarkers for disease. Food industry: Peptide mapping, vitamins, contaminants and toxins. Agriculture: Environmental toxins and polar analytes found in plant extracts. the race online