WebFeb 15, 2024 · African green monkey kidney VeroE6 cells (ATCC CRL-1586) and Crandell-Rees Feline Kidney (CRFK, ATCC CCL-94) cells were maintained at 37 °C with 5% CO 2 in Minimum Essential Medium (MEM; PAN Biotech, Aidenbach, Germany) supplemented with 10% fetal bovine serum (PAN Biotech), 100 IU/mL penicillin G and 100 µg/mL … WebOct 22, 2024 · Crandell-Rees feline kidney cells (CRFK; ATCC) were cultured in T150 flasks (Corning), inoculated with serotype II FIPV (WSU-79-1146; GenBank DQ010921) and propagated in 50 ml Dulbecco’s Modified Eagle’s Medium with 4.5 g/l glucose (Corning) and 10% fetal bovine serum (Gemini Bio). After 72 h of incubation at 37°C, extensive …
Community Resources Council
WebMar 28, 2024 · The cells were maintained at 37°C with 5% CO 2 and passaged every 2–3 days using 0.25% Trypsin-EDTA (ATCC). CRISPR transfection of feline cells Feline CRFK cells were seeded in antibiotic-free, serum-rich EMEM at 250,000 cells/well on six-well plates ∼24 h prior to transfection. WebMay 25, 2013 · Feline Norden Laboratory Feline kidney (NLFK) and Crandell Rees feline kidney (CRFK) cells, canine Cf2TH, A72, and Madin Darby canine kidney (MDCK) cells, human 293 or hamster CHO-K1 cells originated from … induction range near me
兔子Ⅲ型前胶原(HPCⅢ)ELISA试剂盒 - 仪器交易网
WebApr 24, 2001 · Atlanta, GA 30342. 4049490700 [phone] Main Studio Address: 3090 Olive St. Suite 400. Dallas, TX 75219. 2145262400 [phone] Contact via Email. Station Website. WebDec 3, 2008 · Feline infectious peritonitis virus (FIPV) (ATCC-990), an enteric feline coronavirus, was propagated and assayed in the Crandell Reese feline kidney cell line (CRFK) (ATCC-94). Human coronavirus 229E (HCoV) (ATCC-740), a respiratory virus, was propagated and assayed in the fetal human lung fibroblast, MRC-5 cell line (ATCC-171). … WebJun 28, 2013 · Propagation of PhHV-1 was achieved via Crandell Feline Kidney cells (CRFK; ATCC CCL-94), which were kept in EMEM supplemented with 5–10% fetal bovine serum (FBS, Biochrom). A PhHV-1 isolate, designated 2577/88 that was isolated from a diseased harbor seal in 1988 (Dr. Ludwig Haas, Tierärztliche Hochschule Hannover, … logan\u0027s roadhouse promo code