WebOct 9, 2024 · The cycloheximide (CHX) chase assay showed that the DPBA plus CHX treatment lowered the EGFR protein levels considerably more than the CHX treatment … WebCycloheximide (CHX; mol. wt. 281.35) is a beige colored, crystal powdered, water-soluble, semisynthetic compound, ... An alternative is the cycloheximide chase assay, where global protein synthesis is blocked using the translation elongation inhibitor cycloheximide. With this method, the clearance of the protein of interest can be ascertained ...
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WebJan 9, 2024 · Cycloheximide (CHX) chase assay. Cycloheximide (CHX) is a protein synthesis inhibitor in eukaryotes, which is used in cell biology to determine the half-life of a given protein [36, 37]. FLO1 cells with IGFBP2 knockdown and control cells were treated with or without ABS for 20 min and then recovered in full medium with 50 μg/ml CHX. WebSep 11, 2024 · CHX pulse-chase assay was performed to analyze the stability of endogenous SNAI2 protein. (E) Quantification of endogenous SNAI2 protein levels presented in D using ImageJ software. 5 Supplemental Figure S4. ASB13 is a major E3 ligase affecting endogenous SNAI2 protein degradation. chingro
Regulation of c-Myc protein stability by proteasome activator …
WebApr 10, 2024 · A cycloheximide (CHX) pulse-chase experiment was then performed to confirm that OTUD6B stabilizes the ... Cycloheximide Pulse-Chase Assay. For the protein half-life assay, the indicated cells were treated with CHX (50 µg mL −1) for the indicated time points, and subjected to immunoblotting. WebMar 26, 2024 · How to open CHX files. Important: Different programs may use files with the CHX file extension for different purposes, so unless you are sure which format your CHX … WebJul 23, 2024 · In this study, we performed a cycloheximide (CHX) chase assay to measure the half-lives of approximately 30 human lysosomal membrane proteins (LMPs) and identified RNF152 and LAPTM4A as short-lived membrane proteins. The degradation of both proteins is ubiquitin dependent. granite and stone leaders